Dichlorphenolindophenol

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DCPIP
Image:DCPIP-2D-skeletal.png
Systematic name	2,6-dichlorophenol-indophenol
Other names	2,5-cyclohexadien-1-one; dichloroindophenol; 2,6-dichloro-4-[(4-hydroxyphenyl)imino] -2,6-dichloroindophenol; 2,6-Dichloro-4-((4-hydroxyphenyl)imino) -2,5-cyclohexadien-1-one
Chemical formula	C₁₂H₇NCl₂O₂
Molecular mass	268.1 g mol⁻¹
Density	x.xxx g cm⁻³
Melting point	xx.x °C
Boiling point	xx.x °C
CAS number	[956-48-9]
SMILES	O=C(C(Cl)=C2)C(Cl)=C/ C2=N\C1=CC=C(O)C=C1
Disclaimer and references

2,6-dichlorophenolindophenol, or DCPIP is a blue chemical compound used as a redox dye. In its oxidized state DCPIP is blue and when reduced, becomes colorless.

This reaction is reversible, as colorless DCPIP can be reoxidized to blue. It is often used in measurements of the electron transport chain in plants because of its higher affinity for electrons than ferredoxin. It is also commonly used to determine the concentration of ascorbic acid in a solution.

Image:Reduction of dcpip.GIF

[edit] Determining the Concentration of Ascorbic Acid

A solution of DCPIP is made by dissolving the solid in distilled water, the solution is then filtered to ensure purity. This solution is then titrated with the ascorbic acid solution until a faint pink colour persists for 15 seconds indicating the end point of the titration.