Laccase
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Laccases are copper containing oxidase enzymes that are found in many plants, fungi and microorganisms. The copper can be bound in several sites; Type 1, Type 2, and/or Type 3. When types 2 and 3 are bound together, it is called a trinuclear cluster. Laccases are enzymatically active on phenols and similar molecules and perform a one electron oxidation. Laccases can be polymeric and the enzymatically active form can be a dimer or trimer.
The easiest way to detect activity in Laccases is with a spectrophotometer. Substrates that are commonly used with this method are ABTS, 2,6 Dimethoxyphenol and Dimethyl-p-Pheylenediamine.
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