MTT assay
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MTT assay is a laboratory test and a standard colorimetric assay (an assay which measures changes in colour) for measuring cellular proliferation (cell growth).
Yellow MTT is(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a type of tetrazole) reduced to purple formazan and then solubilization solution is added to convert the insoluble purple formazan product into a colored solution which can be detected at 570nm by a spectrophotometer. This reduction takes place only when mitochondrial reductase enzymes are active, and thus conversion is directly related to the number of viable cells. The production of purple formazan in cells treated with an agent is measured relative to the production in control cells, and a dose-response curve can be generated.
[edit] References
- Mosmann, T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J. Immunol. Meth. 1983, 65, 55-63.fr:Test MTT
