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In a RESOLFT concept the resolution can be approximated by <math>\Delta d = \frac{\lambda}{\pi \cdot n\cdot\sqrt{\frac{I}{Isat}}}</math>, whereby Isat is the characteristic intensity required for saturating the transition, and I denotes the intensity applied. If the minima are produced by focusing optics with a numerical aperture nsin α, the minimal distance at which two identical objects can be discerned is <math>\Delta d = \frac{\lambda}{2n\cdot\sin\alpha\cdot\sqrt{1+\frac{I}{Isat}}}</math> which can be regarded as an extension of Abbe’s equation. The diffraction-unlimited nature of the RESOLFT family of concepts is reflected by the fact that the minimal resolvable distance can be continuously decreased by increasing <math>\varsigma = \frac{I}{Isat}</math>. Hence the quest for nanoscale resolution comes down to maximizing <math>\varsigma = \frac{I}{Isat}</math>. This is possible by increasing I or by lowering Isat.

RESOLFT stands for REversible Saturable OpticaL Fluorescence Transitions

[edit] References

• Brief summary of the RESOLFT equations developed by the Max Planck Institute for Biophysical Chemistry.

[edit] See also

• microscope
• STED microscope