Vero cell

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Vero cells are used in cell cultures. Vero cells are cells from the Vero lineage derived from epithelial cells of kidney from African green monkey (Cercopithecus aethiops).<ref name=Yasumura_1963>Yasumura Y, Kawakita M (1963). "The research for the SV40 by means of tissue culture technique". Nippon Rinsho 21 (6): 1201–1219.</ref> The Vero lineage was isolated on 27 Mar 1962, by Yasumura and Kawakita at the Chiba University in Chiba, Japan.

Vero cells are used for many purposes, including:

screening for the toxin of E. coli, first named Vero toxin after this cell line, and later called Shiga-like toxin due to its similarity to Shiga toxin isolated from Shigella dysenteriae.
as host cells for growing virus; for example, to measure replication in the presence or absence of a research pharmaceutical, or testing for the presence of rabies virus.
as host cells for eukaryotic parasites, specially of the Trypanosomatids.

The Vero cell lineage is continuous and aneuploid. A continuous cell lineage can be replicated through many cycles of division and not become senescent.<ref name=ECACC>Main Types of Cell Culture. Fundamental Techniques in Cell Culture: a Laboratory Handbook. Retrieved on 2006-09-28.</ref> Aneuploidy is the characteristic of having an abnormal number of chromosomes.

[edit] Lineages

Vero (ATCC No. CCL-81)

Isolated from C. aethiops kidney on 27 Mar 1962.

Vero 76 (ATCC No. CRL-1587)

Isolated from Vero in 1968.

Vero E6 (ATCC No. CRL-1586)

This line is a clone from Vero 76.

Research strains transfected with viral genes:

Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein Glycoprotein-H (gH).<ref name=Forrester_1992>Forrester A, Farrell H, Wilkinson G, Kaye J, Davis-Poynter N, Minson T (1992). "Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted.". J Virol 66 (1): 341-8. PMID 1309250.</ref> Expression of gH is under the control of the promoter region of HHV-1 entry protein Glycoprotein-D (gD), which is simultaneously transfected via a concatenated plasmid with the gH gene downstream of the gD promoter. (Also F6B2; obs. F6B1.1)

[edit] References

History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf ja:培養細胞